About RecombiMAb anti-mouse CD71 (TfR1) The CP055 monoclonal antibody is a recombinant, chimeric version of the original 8D3 antibody. The variable domain sequences are identical to the original 8D3 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a, kappa for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The anti-tumor and cytotoxic activities of anti-TfR1 antibodies have been demonstrated to require Fc effector function to elicit antibody-dependent cell-mediated cytotoxicity and antibody-dependent cell-mediated phagocytosis. The 8D3 monoclonal antibody reacts with native, soluble and denatured forms of murine CD71, also known as transferrin receptor protein 1 (TfR1). CD71 is a type II homodimeric transmembrane glycoprotein expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. CD71 is overexpressed on many different types of cancer cells and expression level correlates with advanced stage and/or poorer prognosis in several cancers, including solid cancers. Elevated levels of CD71 expression on malignant cells, together with its extracellular accessibility, ability to internalize, and central role in cancer cell pathology make this receptor an attractive target for antibody-mediated therapy. In addition, cells of the vascular endothelium of brain capillaries that compose the blood-brain barrier (BBB) also express high levels of CD71 allowing for receptor-mediated transcytosis of large biomolecules into the brain. Chimeric anti-CD71 clone 8D3 has been used as a BBB transporter in mice and is suitable for studying CD71 expression and iron uptake into different tissues. RecombiMAb anti-mouse CD71 (TfR1) Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)RecombiMAb mouse IgG2a isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenMouse transformed endothelioma cell line t.end1 Reported ApplicationsTargeted drug delivery to the brain Immunohistochemistry Flow Cytometry Western Blot In vivo depletion of CD71+ cells In vivo anti-tumor activity *Reported for the original 8D3 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
