{"id":"557","post_author":null,"post_date":null,"post_date_gmt":null,"post_content":null,"post_title":"InVivoSIM anti-human RANKL (CD254) (Denosumab Biosimilar) ","post_excerpt":"This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Denosumab making it ideal for research use. This Denosumab biosimilar reacts with human RANKL (receptor activator of nuclear factor kappa-B ligand) also known as CD254 and TRANCE (TNF-related activation-induced cytokine). RANKL is a 35 kDa type II membrane protein that belongs to the TNF superfamily. RANKL is expressed on activated T lymphocytes in the lung, thymus, and lymph nodes and on osteoclasts. RANKL has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation. Additionally, RANKL was found to be critical for osteoclast differentiation. Denosumab is an inhibitor of RANKL which functions by decreasing the development of osteoclasts. ","post_status":"publish","comment_status":null,"ping_status":null,"post_password":null,"post_name":"invivosim-anti-human-rankl-cd254-denosumab-biosimilar--sim0028","to_ping":null,"pinged":null,"post_modified":null,"post_modified_gmt":null,"post_content_filtered":null,"post_parent":null,"guid":null,"menu_order":null,"post_type":null,"post_mime_type":null,"comment_count":null,"filter":null,"sku":"SIM0028","path":"\/product\/invivosim-anti-human-rankl-cd254-denosumab-biosimilar--sim0028\/","image":"https:\/\/pneuma.bioxcell.com\/Media\/Products\/bioxcell-sim0028-invivosim-anti-human-rankl-cd254-denosumab-biosimilar.png","brand_id":null,"brand_name":null,"brand_slug":null,"brand_description":null,"isotype":"Human IgG1, \u03ba","immunogen":"Human RANKL","reported_applications":"Functional assays\nFlow Cytometry\n","aggregation":"<5%\nDetermined by SEC","formulation":"PBS, pH 7.0\nContains no stabilizers or preservatives","murine_pathogen_tests":"Mouse Norovirus: Negative\nMouse Parvovirus: Negative\nMouse Minute Virus: Negative\nMouse Hepatitis Virus: Negative\nReovirus Screen: Negative\nLymphocytic Choriomeningitis virus: Negative\nLactate Dehydrogenase-Elevating Virus: Negative\nMouse Rotavirus: Negative\nTheiler's Murine Encephalomyelitis: Negative\nEctromelia\/Mousepox Virus: Negative\nHantavirus: Negative\nPolyoma Virus: Negative\nMouse Adenovirus: Negative\nSendai Virus: Negative\nMycoplasma Pulmonis: Negative\nPneumonia Virus of Mice: Negative\nMouse Cytomegalovirus: Negative\nK Virus: Negative","human_pathogen_tests":"","toxicity_test_results":null,"endotoxin":"<ul><li>\u22640.5EU\/mg (\u22640.0005EU\/\u03bcg)<\/li><li>Determined by LAL gel clotting assay<\/li><\/ul>","purity":"<ul><li>\u226595%<\/li><li>Determined by SDS-PAGE<\/li><\/ul>","sterility":"0.2 \u03bcm filtration","production":"Purified from cell culture supernatant in an animal-free facility","purification":"Protein A","composition":null,"volume":null,"concentration":null,"molecular_weight":"150 kDa","storage":"The antibody solution should be stored at the stock concentration at 4\u00b0C. Do not freeze.","rrid":"","recommended_isotype_control":"<a href=\"\/product\/\">RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme<\/a>","recommended_dilution_buffer":"<a href=\"\/product\/\">InVivoPure pH 7.0 Dilution Buffer<\/a>","clone":"Denosumab","technical_data_sheet":"https:\/\/pneuma.bioxcell.com\/api\/v1\/products\/certificate-of-analysis\/SIM0028\/0\/0\/0?apikey=23D50188E6F74755AFFBF95BD41B140B","western_blot_image":null,"new_product_image":null,"product_references":null,"display_pubmed_logo":null,"modified":"2026-03-18 02:10:05","active":"Y"}

InVivoSIM anti-human RANKL (CD254) (Denosumab Biosimilar)

Clone	Catalog #	Category
Denosumab	SIM0028

USD 235 - USD 8140

Technical Data Sheet

SDS Sheet

About InVivoSIM anti-human RANKL (CD254) (Denosumab Biosimilar)

This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Denosumab making it ideal for research use. This Denosumab biosimilar reacts with human RANKL (receptor activator of nuclear factor kappa-B ligand) also known as CD254 and TRANCE (TNF-related activation-induced cytokine). RANKL is a 35 kDa type II membrane protein that belongs to the TNF superfamily. RANKL is expressed on activated T lymphocytes in the lung, thymus, and lymph nodes and on osteoclasts. RANKL has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation. Additionally, RANKL was found to be critical for osteoclast differentiation. Denosumab is an inhibitor of RANKL which functions by decreasing the development of osteoclasts.

InVivoSIM anti-human RANKL (CD254) (Denosumab Biosimilar) Specifications

Isotype	Human IgG1, κ
Recommended Isotype Control(s)	RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Immunogen	Human RANKL
Reported Applications	Functional assays Flow Cytometry
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	≥95% Determined by SDS-PAGE
Sterility	0.2 μm filtration
Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein A
Molecular Weight	150 kDa
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.