About RecombiMAb anti-mouse EGFR (domain-II) The EMab-301-CP068 monoclonal antibody is a recombinant version of the original EMab-301 antibody. The variable domain sequences are identical but the constant region sequences have been converted from rat IgM to rat IgG2a. EMab-301-CP068 selectively binds extracellular domain II of mouse EGFR (epidermal growth factor receptor). Domain II is a critical segment of the receptor's extracellular region responsible for mediating receptor dimerization and activation upon ligand binding. Mouse EGFR (also known as ERBB, ERBB1, and HER1) is a transmembrane glycoprotein and a member of the ErbB family of receptor protein tyrosine kinases (RTKs). EGFR ligands include EGF, AREG, TGF-α, epiregulin (EREG), epigen, betacellulin, and heparin-binding EGF. Upon ligand binding, EGFR dimerizes and is activated through autophosphorylation of the cytoplasmic domain. EGFR activation triggers several downstream signaling pathways, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC-γ-PKC, and STATs which help the cell respond accurately to extracellular signals. EGFR is expressed ubiquitously in various tissues and plays critical roles in developmental, physiological, and regenerative processes. The aberrant activation of EGFR is a common feature of various malignancies. RecombiMAb anti-mouse EGFR (domain-II) Specifications IsotypeRat IgG2a, κ Recommended Isotype Control(s)InVivoPlus rat IgG2a isotype control, anti-trinitrophenol Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer Immunogenmouse EGFR overexpressing cells Reported ApplicationsWestern Blot For information on applications, please contact (technicalservice@bioxcell.com) FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from mammalian cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
