RecombiMAb anti-mouse IL-10R (CD210)

About RecombiMAb anti-mouse IL-10R (CD210)

The 1B1.3A-CP073 monoclonal antibody is a recombinant, chimeric version of the original 1B1.3A antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG1, κ to mouse IgG2a, κ for use in murine models. Additionally, 1B1.3A-CP073 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine FcγR or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). Antibodies with active Fc regions can engage immune cells via FcγRs, leading to the depletion of antigen expressing cells through mechanisms like ADCC or complement activation. Fc-silenced antibodies do not trigger these pathways and can block signaling without killing or depleting target cells. Using Fc-silent antibodies allows researchers to distinguish between the effects of receptor blockade and those of cell depletion or Fc-mediated immune activation. Additionally, species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. 1B1.3A-CP073 monoclonal antibody reacts with mouse IL-10R (IL-10 receptor) also known as CD210. The IL-10R is a class II cytokine receptor and is expressed by a variety of cell types including thymocytes, T lymphocytes, B lymphocytes, NK cells, monocytes, and macrophages. Upon binding IL-10, IL-10R stimulation results in many pleiotropic effects in immunoregulation and inflammation. IL-10R downregulates the expression of pro-inflammatory cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B lymphocyte survival, proliferation, and antibody production. IL-10R signaling can block NF-κB activity and is involved in the regulation of the JAK-STAT signaling pathway. The 1B1.3A antibody is a neutralizing antibody and has been shown to block the binding of human IL-10, which cross-reacts with the mouse IL-10R. However, this clone does not recognize the human IL-10R.